Mycobacterium tuberculosis?
Im looking for detailed info on growing M tuberculosis; I know that Lowenstein-Jensen slants are used, but I can't find anything that says what they are etc. Links/refrences would be great. Oh, and no wikipedia: if nothing else i drew a blank there. haha. Im starting a research project in a few weeks for my degree, and im going to be looking for potential new targets for drugs, which is all the more important with the spread of Extreme drug resistance TB
Biology - 5 Answers
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1 :
wow, some serious s hit - u wanna know how to grow this why> or u need it for a report.
2 :
Bacteria boy Try this location, and could you put in a PS Explaining what in the heck are you going to do with this culture of a deadly Bacterium? Possibly start your own epidemic? if you are let us know so we can buy stock in the company that makes the anti bacterial that is most effective http://www.personal.psu.edu/faculty/w/x/wxm15/Online/Bacteriology/bacteriology_lec00.
3 :
Mycobacterium - a genus of rod-shaped acid-fast bacteria. Tuberculosis - a specific infective disease caused by the bacteria ( mycobacterium). Some links or reference: 1. www sanger.ac.uk/Projects/m_tuberculosis 2. www micro.msb.le.ac.uk/video/mtuber.html
4 :
A good starting point is: Biosafety in Microbiological and Biomedical Laboratories, a joint publication of the CDC/NIH. Your next stop should be a major University Hospital medical library. The librarian will help you find the most recent publication on cultures and propagation and testing for drug resistance. Then visit the Hospital Lab where this is done.
5 :
Several media both solid and liquid have been described for the growth of tubercle bacilli Solid media contain egg (Lowenstein-jensen, Petragnini,Dorset egg medium Blood (Tarshis), Serum (Leoffler's serum slope), Potato (Pawlowsky) The medium most commonly used is Lowenstein Jensen's medium W/o starch. This contains coagulated Hen's egg, mineral salt solution, asparagine,and malachite green, the last acting as selective agent inhibiting other bacteria. Liquid media include- Dubos',Middlebrook's,Proskauer and Beck's. liquid media are generally not used for routine cultivation, but are used for sensitivity testing, chemical analyses and preparation for antigens and vaccines The bacilli grow slowly, the generation time in vitro being 14-15 hrs. Colonies appear in about 2 weeks, and may sometimes take upto 8 weeks. Optimum temp is 37 C and growth does not occur below 20C or 40 C.Optimum pH IS 6.4-7. On solid media M. Tuberculosis forms dry, rough raised irregular colonies with a wrinkled surface. They are creamy white, becoming yellowish or buff coloured on further incubation.They are tenacious and not easily emulisified. In liquid media without dispersing agents growth begins at the bottom,creeps up the sidesand forms a prominent surface pellicle which may extend along the sides above the medium. Diffuse growth is obtained in Dubo's medium containing Tween 80. Virulent strains tend to form serpentine cords in liquid media, while avirulent strains grow in a more dispersed manner
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