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Broth and slant properties? I hope this is what you are looking for... Mycobacteria tuberculosis (M. tb) grows well on Lowenstein-Jensen media (LJ media), which is an egg based media that contains malachite green (among other chemicals) to inhibit other types of bacterial growth. It also grows well on a Middlebrook medium(labeled 7H-10 or 7H-11). It grows slowly, if at all, on Blood Agar. Colonies are non-pigmented, roughish and raised on the various media. Under a microscope, the colonies appear similar to a cross-section of a brain, or a cauliflower... For broths, M. tb grows best in Middlebrook (7H-9) broths, but can also grow in Sodium thiosulfate broths as well. Colonies have the same appearance as they do on solid media, although they do float in clumps in liquid media. M. tb grows at 25-37 degrees Celsius, although it prefers 35 degrees. Growth is enhances slightly with CO2, depending on the specific strain. Hope this helps! This info pertains only to Mycobacterium tuberculosis, other types of mycobacteria have different characteristics. Check the CDC website for pictures and further information: www.cdc.gov
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