what would be a good method for identifying the bacteria, which causes leprosy or tuberculosis?
Biology - 2 Answers
Random Answers, Critics, Comments, Opinions :
1 :
DNA testing of samples. Can't grow M lerprae, and M. tuberculosis takes a very, very long time to culture.
2 :
ACID FAST STAINING it is similar in concept to gram staining, but it seperates bacteria into two colors based on whether they're in the genus Mycobacterium (leprocy/Tuberculosis) versus all other genera. step 1: apply basic carbolfuchsin dye which is the primary dye, it will stain all cells red but binds stronger to cell wall rich in a certain wax step 2: wash slide with acid alcohol to decolorize bacteria lacking waxy material step 3: apply basic stain methylene blue to color all unstained cells blue, this is a counterstain when all is said and done, the acid fast cells (Mycobacterium) are red, all others are blue hope that helps
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